The nut seeds of Garcinia kola are traditionally used in African medicine and known for their anti-microbial, anti-oxidative and anti-inflammatory activities. Long-chain metabolites of α-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro. Dr. Mosig was a researcher I got to know Since 2015 Elected member of the Board of Directors of the CSCC. The actin cytoskeleton is central for cellular homeostasis and is targeted for anti-cancer chemotherapy. Email: [email protected] Phone: +49 3641-9-47330. In an earlier study, we identified multiple genes as hypermethylated in tumors of patients with poor prognosis. Chemisch-Geowissenschaftliche Fakultät der Universität Jena, Unter dem Dach der Chemisch-Geowissenschaftlichen Fakultät sind an der Friedrich-Schiller Universität Jena die Fachrichtungen Chemie, Geologie, Mineralogie, Geophysik und Geographie vereint. Gröger M(1), Dinger J(2), Kiehntopf M(3), Peters FT(2), Rauen U(4), Mosig AS(1). Title: Microsoft Word - CV Mosig short eng.docx Created Date: 7/25/2018 5:51:58 PM The influenza virus (IV) can cause severe epidemics, which results in significant morbidity and mortality. In the presence of ECs a lipopolysaccharide (LPS)-stimulus led to a significant increase... Liver dysfunction is an early event in sepsis-related multi-organ failure. Address: 1Molecular Hemostaseology, Friedrich-Schiller-University of Jena, Bachstrasse 18, 07743 Jena, Germany, 2Institute of Molecular Cell University of Jena Teaching Research Team Faculty Back Team Microeconomics Team Team Information about our team. The most promising combination of markers to predict a patient’s outcome was CaMKIINα and RUNX3. April 2020 In case of equal qualification, persons with disabilities will be given priority. Institut für Biochemie II Universitätsklinikum Jena Nonnenplan 2-4 07743 Jena Telefon: +49 3641 9 390 910 Telefax: +49 3641 9 396 402 [email protected] We show that FKN triggers different effects in CD16(+) and CD16(-) monocytes, the two major subsets of human monocytes. News. Friedrich Schiller University, Institute of Microbiology. We will see you in Jena in the winter semester 2020/21—partly on site, partly online 10 July 2010 10 July 2010 Educational operations of the winter semester 2020/21 at the Friedrich Schiller University will start on 2 November 2020 and will be conducted—at least partially—on site. or write an Email to [email protected] Reference number: DM23/2020 (internal and external) Application deadline: 30. Press, A. T., et al. Particle Formulation Group (PFG) Jena Center for Soft Matter (JCSM) Friedrich-Schiller-Universität Jena Philosophenweg 7 07743 Jena Telefon: +49 3641 9 48 599 Genome-wide analyses of hypermethylated CpG-islands in resistant cell lines in combination with qRT-PCR analyses were used to identify epigenetically silenced genes. The development of therapeutic substances to treat diseases of the central nervous system is hampered by the tightness and selectivity of the blood-brain barrier. Hepatic α-TOH catabolism results in intermediate formation of long-chain metabolites (α-LCMs(5)) α-13'-hydroxy- and α-13'-carboxychromanol (α-13'-OH(6) and α-13'-COOH(7)). We here report the establishment of a liver organoid that integrates NPCs in a vascular layer composed of endothelial cells and tissue macrophages and a hepatic layer comprising stellate cells co... We have established an electroporation protocol for transfection of premature adherent human THP-1 macrophages using Lonza Nucleofector technology. A prerequisite for clonal expansion of cancer cells is unlimited proliferative capacity. (2015). Mechanical stimulation of the endothelial monolayer induces morphological remodeling in its cytoskeleton... Hemodynamic forces generated by the blood flow are of central importance for the function of endothelial cells (ECs), which form a biologically active cellular monolayer in blood vessels and serve as a selective barrier for molecular permeability. It cross-links the activities of its principal and visiting scientists together with around 300 doctoral and more than 150 Master's degree students. Search for more papers by this author. E‐mail: [email protected]‐ Search for more papers by this author 07743 Jena Site plan +49 3641 9-43000 +49 3641 9-43002 [email protected] Opening hours: By appointment Students Junior scientists Staff Alumni & Patrons Researchers Share this page. Despite intensive research the physiological role and molecular mechanisms of action of the lipophilic antioxidant α-tocopherol (α-TOH(4)) are still poorly understood. Here, we established a new microfluidically supported, biochip-based model of the brain endothelial barrier in c... Fractalkine (FKN, CX3CL1) is a regulator of leukocyte recruitment and adhesion, and controls leukocyte migration on endothelial cells (ECs). Increased CD36 and CD91/Lrp1 levels on the surface of monocytes from FH patients and their role in LDL uptake. Within the liver, non-parenchymal cells (NPCs) are critically involved in the regulation of hepatocyte polarization and maintenance of metabolic function. International Organizations and Crisis Management - IOCM in short - is the masters degree in political science of the Chair for International Relations at the Friedrich-Schiller-University Jena. Identifikation von Hochfettdiät-induzierten inflammatorischen Gennetzwerken assoziiert mit der Suppression anti-inflammatorischer Phospholipide in humanem Fettgewebe in der NUGAT (NutriGenomics Analysis in Twins) Studie, Sensor enhanced microfluidic devices for cell based assays and organs on chip. There are no tuition fees at the Friedrich Schiller University Jena. For more information please contact PD Dr. Alexander Mosig A: legend of array index. [email protected] The study was undertaken to investigate whether the two major monocyte subsets defined by the surface markers CD14(+)CD16(+) and CD14(++)CD16(-) show differences in their responses to hypercholesterolemia. The intestine is the primary reservoir of Candida albicans that can cause systemic infections in immunocompromised patients. Marko Gröger. [email protected] All rights reserved. Although the viral infection originates in the respiratory system, it is unclear how the virus can overcome the alveolar barrier, which is observed in severe COVID-19 disease courses. It has been suggested that taurolidine has no severe side-effects, but after long-term use morphological and functional changes of the liver were reported. Since 2015 Head of Research Group INSPIRE – In Vitro Sepsis Research, Center for Sepsis Control and Care . Petra joined Jena University Hospital (JUH), Department of Anaesthesiology and Intensive Care Medicine, in November 2016 as senior academic clinical lecturer to connect her research interests and expertise with the infection research at JUH and its integrated research and treatment Centre for Sepsis Control and Care (CSCC). 30 Sep 2020. We sought to examine effects of a high‐saturated‐fat (HF) diet on ACE within the NUtriGenomic A... Abstract Near infrared spectroscopy (NIRS) is a rapid and non-destructive method for compositional analysis of biological material. Statin therapy has no effect of SREBP gene expression in monocytes. Expression of NPC1 and ABCA1 in monocytes of FH patients and of healthy control individuals. Long-term overseas dispatch program in Friedrich Schiller University Jena D2 Kaori Machitani Takeda 1. (2009). Cigarette smoke components have a proven negative influence on human health. Henceforth, it is possible to proceed the studies or to start the studies without being physically in Jena. In general, surfactant protein-A (SP-A) represents a first line of immune defense. (2) ... 07747 Jena, Germany. Memberships. Learn more. Analyses of gene and protein expression by qRT-PCR and Western Blot reveal down-regulation of members of the SREBP-pathway. Monocytes were rapidly isolated from the blood of hypercholesterolemic, low-density lipoprotein (LDL) receptor-defective familial hypercholester... Elevated plasma cholesterol promotes the formation of atherosclerotic lesions in which monocyte-derived lipid-laden macrophages are frequently found. Jena University Hospital Center for Sepsis Control and Care Dr. Alexander Mosig, Email: alexander.mosig[at], Phone +49 03641-9346418 References 1. ); The purpose of the present study was to investigate the effects of acute exercise on gene expression profiles (GEPs) of WBCs and to identify suitable genes that may serve as surrogate markers for monitoring exercise and... Ziel dieser Arbeit war es, anhand der Untersuchung von Genexpressionsprofilen (GEP) von Monozyten und T-Zellen weitergehende Erkenntnisse über die molekularen und zellulären Vorgänge in diesen Zellen bei der Entwicklung der Atherosklerose von Patienten mit Familiärer Hypercholesterinämie zu erhalten. human liver sinusoid. Societies Identification of high fat diet induced inflammatory gene networks associated with suppression of anti-inflammatory phospholipids in human adipose tissue in the NUGAT (Nutrigenomics Analysis in Twins) study, Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, A microfluidically perfused three dimensional human liver model, Optimization of the transfection of human THP-1 macrophages by application of Nunc UpCell technology, Cell type-specific delivery of short interfering RNAs by dye-functionalised theranostic nanoparticles, High fat diet induced adipose tissue inflammation by suppression of anti-inflammatory phospholipids, Identification of gene-networks associated with specific lipid metabolites by Weighted Gene Co-Expression Network Analysis (WGCNA), Microfluidic devices for cell culture and handling in organ-on-a-chip applications, Long-chain metabolites of α-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro, SORBS2 and TLR3 induce premature senescence in primary human fibroblasts and keratinocytes, Analysis of glucose and fetal calf serum in aqueous solution for Multi Organ Tissue Flow (MOTiF) bioreactors using NIR Spectroscopy, Towards an understanding of the interaction between liver cells and antibiotics via Raman microspectroscopy, Antioxidative and Non-Antioxidative Effects of Tocopherol Metabolites on Atherogenesis, METHOD AND DEVICE FOR DETECTING THE MOTION AND ADSORPTION OF CELLS AND PARTICLES ON CELL, TISSUE, AND IMPLANT LAYERS IN THE SIMULATION OF FLOW CONDITIONS, Verfahren und Vorrichtung zur Erfassung der Bewegung und Anlagerung von Zellen und Partikeln an Zell-, Gewebe- und Implantatschichten bei der Simulation von Flussbedingungen, Gene Expression And Autologous Blood Transfusion - A New Advance Towards Blood Doping Detection? Editorial. © 2008-2020 ResearchGate GmbH. Gerhard G. Paulus. 17 6 Center for Electron Microscopy, Jena University Hospital, Ziegelmuehlenweg 1, D-07743 Jena, Germany 18 19 20 * Correspondence: Dr. Stefanie Deinhardt-Emmer 21 Institute of Medical Microbiology, 22 Jena University Hospital, 23 Am Klinikum 1, 24 D-07747 Jena, Germany 25 26 Email: [email protected] The Faculties of Friedrich Schiller University Jena The University is organised into ten Faculties: The system is based on concepts of modular development, rapid-prototyping and all-time accessibility using widely available off-the-shelf optic and electronic components. Alexander S. Mosig1,2 1Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany 2Institute of Biochemistry II, Jena University Hospital, Jena, Germany 3fzmbResearchCentreofMedical Technology and Biotechnology, Bad Langensalza, Germany 4Arthrospec GmbH, Erfurt, Germany Research Article Novel approach for the prediction of cell Image: Jan-Peter Kasper (University of Jena) The Abbe Center of Photonics (ACP) hosts fundamental and applied research in optics and photonics. Functional Particles (FP) Research topic. B: Whisker box plots of log2 transformed microarray raw data. The fluorescent dye, even when coupled to a nanoparticle, mimics... For many problems in system biology or pharmacology, in-vivo-like models of cell-cell interactions or organ functions are highly sought after. Analysis of gene expression by qRT-PCR for CD36 and LRP1. Preparation, modification and characterization of polymeric nanoparticles for targeted drug delivery applications Contrary to the assumption that ACE levels are stable, circulating ACE has been shown to be altered in obesity and weight loss. We t... White blood cells (WBCs) express tens of thousands of genes, whose expression levels are modified by genetic and external factors. Molecular Hemostaseology, Friedrich‐Schiller‐University of Jena, Jena, Germany. Mit dem Benutzen der Webseite erklären Sie sich der Nutzung von Cookies einverstanden. The City of Jena. Marko Gröger. Author information: (1)Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany. The Functional Photonic Nanostructures junior research group at the Institute of Applied Physics is dedicated to the study of the fundamental optical properties and potential applications of subwavelength … Subgroup. Raman spectroscopic imaging was used to visualize the cell nuclei by means of false color imaging using a marker band, and a cell segmentation w... Complex cell culture models such as microphysiological models (MPS) mimicking human liver functionality in vitro are in the spotlight as alternative to conventional cell culture and animal models. The aim was to detect the motion processes and adsorption processes of cells and particles in the simulation of flow conditions completely and more precisely. Delivery&Parcel Address. In this work, Raman spectroscopic cell imaging approaches and a discrimination between HepG2, nondifferentiated hepatic stem cell line HepaRG, and differentiated hepatocyte‐like HepaRG cells are presented. establishment and characterization of a microfluidically supported in vitro organoid model of the Conventional stationary cell culture in 2D plates quickly reaches its limitations with respect to an in-vivo like expression and function of individual cell types. Studies on Franci... Mitochondria contribute to redox and calcium balance, and apoptosis thus regulating cellular fate. These authors contributed equally to this work.Correspondence: Bachstrasse 18, 07743 Jena, Germany. Institute Research The liver is a central organ in the metabolization of nutrition, endogenous and exogenous substances, and xenobiotic drugs. cells Article VEGF Triggers Transient Induction of Autophagy in Endothelial Cells via AMPK 1 Katrin Spengler 1, Nderim Kryeziu 1, Silke Große 1, Alexander S. Mosig 2 and Regine Heller 1,* 1 Institute of Molecular Cell Biology, Center for Molecular Biomedicine, Jena University Hospital, 07743 Jena, Germany; [email protected] (K.S. Learn more. Histogram bars indicate the mean ± SD. Aim was to identify methylated genes with functional involvement in cisplatin-resistance development of epithelial ovarian cancer (EOC). T lymphocytes get in contact with affected organs (e.g., lung) or cells (e.g., erythrocytes), as well as with smoke components and bioactive molecules, whose production is triggered by tobacco smoke. Sorry, you need to be a researcher to join ResearchGate. Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. The data provided show that NPC1 as well as ABCA1 expression is deceased in FH monocytes. Learn more. Here, we... Background Angiotensin‐converting enzyme (ACE) plays a major role in blood pressure regulation and cardiovascular homeostasis. CD36 mediates uptake of oxLDL in monocytes of FH patients and healthy individuals. The emerging organ-on-chip technology has paved the way to model essential liver functions as well as certain aspects of liver disease in vitro in liver-on-chip models. Histogram bars indicate the mean ± SD (* p < 0.05, ** p < 0.01). sebastiano.bernuzzi[AT] Theoretisch-Physikalisches Institut Friedrich-Schiller-Universitaet Jena Max Wien Platz 1 D-07743 Jena, Germany Office 209. Microfluidically supported biochip design for culture of endothelial cell layers With improved perfusion conditions. [email protected] +49 3641 9-48516 Room K018 Fraunhoferstraße 6 07743 Jena Professorship of Bioactive Glasses Site plan Image: Delia Brauer. The toolboxes are named according to the SPM version. Cell type-specific delivery of short interfering RNAs by dye-functionalised theranostic nanoparticles. 3, Room 5.11, 5th floor Phone: + 49 (0) 3641 / 9-43230 Fax: + 49 (0) 3641 / 9-43232 Email: [email protected] Office hours In the present study, mitochondrial staining applying a novel dye, V07-07059, was performed in human embryonic kidney cells, a human vascular endothelial cell line and primary human mononuclear cells. For postdocs and staff, there … We here report the Tumour-associated macrophages mainly comprise immunosuppressive M2 phenotypes that promote tumour progression besides anti-tumoural M1 subsets. A. Mosig, Algorithmen und Datenstrukturen zur effizienten Konstellationssuche (in German, Algorithms and data structures for efficient constellation searching), Master's thesis, Univ. (2)Institute of Forensic Medicine, Jena University Hospital, 07747, Jena, Germany. Here, we describe highly specific nanoparticles, based on near infrared fluorescent polymethine dye-derived targeting moieties coupled to biodegradable polymers. PD Dr. Alexander Mosig Institute for Biochemistry II und Center for Sepsis Control and Care, Universitätsklinikum Jena „Human microphysiological systems as in vitro platform for emulation of gut, liver, and lung in disease modelling and infection research “ Wednesday Feb 20th, 11:15 BFS, Seminar Room 17.1/2 Schubertstr. joachim [dot] denzler [at] uni-jena [dot] de: Phone: +49 3641 946420: Consultation hour: Monday, 2-3 pm (during the lecture periods) Postal Address. Raasch M, Rennert K, Jahn T, Peters S, Henkel T, Huber O, Schulz I, et al. To create a platform for the investigation of the underlying mechanisms, we established a three-dimensional micro... Sepsis is a leading cause of mortality in the critically ill, characterized by life-threatening organ dysfunctions due to dysregulation of the host response to infection. Last update: June 2, 2020 Due to the Corona pandemic, the courses in the current summer term 2020 and also in the upcoming winter term 2020/2021 will be online courses. Jena University Hospital Center for Sepsis Control and Care Dr. Alexander Mosig, Email: alexander.mosig[at], Phone +49 03641-9346418 References 1. Learn more. We show that VEGF lead... With UC2 (You-See-Too) we present an inexpensive 3D-printed microscopy toolbox. Adverse metabolic effects were observed in tissues and single cells. non-parenchymal cells closely reflecting tissue archit... Background Quality control of microarry raw data. With UC2 (You-See-Too) we present an inexpensive 3D-printed microscopy toolbox. ); [email protected] (N.K. Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute. ABCA1 expression is further decreased during monocyte to macrophage differentiation of FH monocytes compared to monocytes of healthy individuals. Liposomes represent suitable tools for the diagnosis and treatment of a variety of diseases, including cancers. Image: Jan-Peter Kasper (University of Jena) Service. Central institutions and faculties of Jena University. The new fluorescent mega Stokes dye (peak excitati... Polymer-based nanoparticles are promising drug delivery systems allowing the development of new drug and treatment strategies with reduced side effects. Different functions of monocyte subsets in familial hypercholesterolemia: potential function of DC14+ CD16+ monocytes in detoxification of oxidized LDL. Room: 08N05 Phone: +49-3641-9-46480 Infections with SARS-CoV-2 lead to mild to severe coronavirus disease-19 (COVID-19) with systemic symptoms. Infections with SARS-CoV-2 lead to mild to severe coronavirus disease-19 (COVID-19) with systemic symptoms. The Abbe School of Photonics (ASP) provides education programs with multidisciplinary coverage of optical and photonics technology field topics, ranging from current scientific aspects to engineering, with product applications possible in major economic sectors such as information and communication technologies, medicine and health, manufacturing and energy consumption. [email protected] For efficient electroporation detachment of adherent cells is necessary. (2014). Jena University Hospital, Center for Sepsis Control and Care. Search for more papers by this author. The centers of the boxes represent the median of the genes, upper and lower ends of the boxes represent the 75th and 25th quartiles, respectively.